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ACADM antibody

Anti-ACADM antibody used in Western Blot (WB). GTX100487
Anti-ACADM antibody used in IHC (Paraffin sections) (IHC-P). GTX100487
Anti-ACADM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100487
Anti-ACADM antibody used in IHC (Paraffin sections) (IHC-P). GTX100487

Cat. No. GTX100487

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ACADM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Bovine, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.74 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ACADM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949558

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

acyl-CoA dehydrogenase medium chain , ACAD1 , MCAD , MCADH

Cellular Localization

Mitochondrion matrix

Background

This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

  • Gene ID: 34 ACADM
  • UniProt: P11310 ACADM

Research Area

DATA IMAGES

Anti-ACADM antibody used in Western Blot (WB). GTX100487

GTX100487 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX100487 diluted at 1:1000

Anti-ACADM antibody used in IHC (Paraffin sections) (IHC-P). GTX100487

GTX100487 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DLD1 xenograft, using ACADM(GTX100487) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ACADM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100487

GTX100487 ICC/IF Image

ACADM antibody detects ACADM protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ACADM protein stained by ACADM antibody (GTX100487) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-ACADM antibody used in IHC (Paraffin sections) (IHC-P). GTX100487

GTX100487 IHC-P Image

ACADM antibody detects ACADM protein at mitochondria in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ACADM antibody (GTX100487) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169