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COX4 antibody

KO/KD Orthogonal
Anti-COX4 antibody used in Western Blot (WB). GTX114330
Anti-COX4 antibody used in Western Blot (WB). GTX114330
Anti-COX4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114330
Anti-COX4 antibody used in IHC (Paraffin sections) (IHC-P). GTX114330
Anti-COX4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114330
Anti-COX4 antibody used in Western Blot (WB). GTX114330
Anti-COX4 antibody used in Western Blot (WB). GTX114330
Anti-COX4 antibody used in Western Blot (WB). GTX114330
Anti-COX4 antibody used in Western Blot (WB). GTX114330
Anti-COX4 antibody used in IHC (Paraffin sections) (IHC-P). GTX114330
Anti-COX4 antibody used in IHC (Paraffin sections) (IHC-P). GTX114330
Anti-COX4 antibody used in IHC (Paraffin sections) (IHC-P). GTX114330
Anti-COX4 antibody used in IHC (Paraffin sections) (IHC-P). GTX114330

Cat. No. GTX114330

Reference 31
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all COX4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

mouse muscle , rat muscle , 293T

Predict Reactivity

Rabbit, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human COX4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10625002

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome c oxidase subunit 4I1 , COX IV-1 , COX4 , COX4-1 , COXIV , COXIV-1

Cellular Localization

Mitochondrion inner membrane

Background

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-COX4 antibody used in Western Blot (WB). GTX114330

GTX114330 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (GTX114330) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COX4 antibody used in Western Blot (WB). GTX114330

GTX114330 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (GTX114330) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COX4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114330

GTX114330 ICC/IF Image

COX4 antibody detects COX4 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: COX4 stained by COX4 antibody (GTX114330) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-COX4 antibody used in IHC (Paraffin sections) (IHC-P). GTX114330

GTX114330 IHC-P Image

COX4 antibody detects COX4 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
COX4 stained by COX4 antibody (GTX114330) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-COX4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114330

GTX114330 ICC/IF Image

COX4 antibody detects COX4 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: COX4 stained by COX4 antibody (GTX114330) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-COX4 antibody used in Western Blot (WB). GTX114330

GTX114330 WB Image

Sample (50 μg of whole cell lysate)
A: rat muscle
12% SDS PAGE
GTX114330 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COX4 antibody used in Western Blot (WB). GTX114330

GTX114330 WB Image

Orthogonal

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (GTX114330) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COX4 antibody used in Western Blot (WB). GTX114330

GTX114330 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (GTX114330) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COX4 antibody used in Western Blot (WB). GTX114330

GTX114330 WB Image

Orthogonal

The data was published in the journal Oxid Med Cell Longev in 2019.PMID: 31249652

Anti-COX4 antibody used in IHC (Paraffin sections) (IHC-P). GTX114330

GTX114330 IHC-P Image

The data was published in the journal Nat Commun in 2017. PMID: 29233981

Anti-COX4 antibody used in IHC (Paraffin sections) (IHC-P). GTX114330

GTX114330 IHC-P Image

The data was published in the journal Nat Commun in 2017. PMID: 29233981

Anti-COX4 antibody used in IHC (Paraffin sections) (IHC-P). GTX114330

GTX114330 IHC-P Image

The data was published in the journal Nat Commun in 2017. PMID: 29233981

Anti-COX4 antibody used in IHC (Paraffin sections) (IHC-P). GTX114330

GTX114330 IHC-P Image

The data was published in the journal Nat Commun in 2017. PMID: 29233981

REFERENCE

Application Reference

Sun X et al. Nat Commun 2023; 14 (1) : 7610 Mirabegron displays anticancer effects by globally browning adipose tissues.
Lee H et al. Nat Commun 2023; 14 (1) : 6721 The mitochondrial fusion protein OPA1 is dispensable in the liver and its absence induces mitohormesis to protect liver from drug-induced injury.
Cardile A et al. Int J Mol Sci 2023; 24 (2) Hyperforin Elicits Cytostatic/Cytotoxic Activity in Human Melanoma Cell Lines, Inhibiting Pro-Survival NF-κB, STAT3, AP1 Transcription Factors and the Expression of Functional Proteins Involved in Mitochondrial and Cytosolic Metabolism.
Seki T et al. Nature 2022; 608 (7922) : 421-428 Brown-fat-mediated tumour suppression by cold-altered global metabolism.
Zhewen Zhang et al. Curr Issues Mol Biol 2022; 44 (6) : 2759-2771 Parkin, as a Regulator, Participates in Arsenic Trioxide-Triggered Mitophagy in HeLa Cells.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

COX4 antibody Cat. No. GTX114330

Rating
( Average 4.7 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 4 based on 1 users reviews)
Anti-COX4 antibody used in Western Blot (WB). GTX114330
Date : Anonymous submitted on 12-Sep-2023
Score :
Application Tested : WB
Sample Species : Ms
Sample : mouse liver and brown adipose tissue
Amount used : 30μg
Blocking : Blocking One, 25°C, 1Hr
Primary Antibody : 1:500 and 1:1000, 25°C, 1Hr
Anti-COX4 antibody used in Western Blot (WB). GTX114330
Date : Anonymous submitted on 12-May-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : Synapotosome isolated from mouse brain tissue
Amount used : 10-20 μg
Blocking : 3% milk powder in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 8Hr
Figure Note : Numbers indicated separate mice; L: left and R: right hippocampus and prefrontal cortex (PFC).
Anti-COX4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114330
Date : Anonymous submitted on 09-Dec-2013
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : striatal progenitor cells
Blocking : 2% BSA, 2% normal goat serum in PBS, 25°C, 1.5Hr
Fixation : 4% paraformaldehyde, 4% sucrose in PBS
Permeabilization /Antigen retrieval : 0.05% NP40/PBS
Primary Antibody : 1:250, 4°C, 18Hr
Figure Note : GTX114330(Red),Hoechst 33258(Blue)
Submit a Review
Application Reference
Sun X et al. Nat Commun 2023; 14 (1):7610 Mirabegron displays anticancer effects by globally browning adipose tissues.
Lee H et al. Nat Commun 2023; 14 (1):6721 The mitochondrial fusion protein OPA1 is dispensable in the liver and its absence induces mitohormesis to protect liver from drug-induced injury.
Cardile A et al. Int J Mol Sci 2023; 24 (2) Hyperforin Elicits Cytostatic/Cytotoxic Activity in Human Melanoma Cell Lines, Inhibiting Pro-Survival NF-κB, STAT3, AP1 Transcription Factors and the Expression of Functional Proteins Involved in Mitochondrial and Cytosolic Metabolism.
Seki T et al. Nature 2022; 608 (7922):421-428 Brown-fat-mediated tumour suppression by cold-altered global metabolism.
Zhewen Zhang et al. Curr Issues Mol Biol 2022; 44 (6):2759-2771 Parkin, as a Regulator, Participates in Arsenic Trioxide-Triggered Mitophagy in HeLa Cells.
Application : WB
Reactivity : Human
Chen CY et al. Sci Rep 2021; 11 (1):2377 Cardiac-specific microRNA-125b deficiency induces perinatal death and cardiac hypertrophy.
Application : WB
Reactivity : Mouse
Tsukahara T et al. Cell Signal 2021; 82:109951 Adenine nucleotide translocase 2, a putative target protein for 2-carba cyclic phosphatidic acid in microglial cells.
Szu-Yi Chou et al. Front Cell Dev Biol 2020; 8:564641 Altered Insulin Receptor Substrate 1 Phosphorylation in Blood Neuron-Derived Extracellular Vesicles From Patients With Parkinson's Disease.
Application : WB
Reactivity : Human
Lee H et al. J Biol Chem 2020; 295 (19):6543-6560 The short variant of optic atrophy 1 (OPA1) improves cell survival under oxidative stress.
Mohd Salman et al. Dis Model Mech. 2020; 13 (8):dmm045021 Nrf2/HO-1 mediates the neuroprotective effects of pramipexole by attenuating oxidative damage and mitochondrial perturbation after traumatic brain injury in rats.
Application : WB
Reactivity : Rat
Tan C et al. J Cell Mol Med 2020; 24 (12):6833-6845 Seizure-induced impairment in neuronal ketogenesis: Role of zinc-α2-glycoprotein in mitochondria.
Application : WB
Reactivity : Rat
Shen M et al. Biomed Pharmacother 2020; 124:109911 Total glucosides of paeony decreases apoptosis of hepatocytes and inhibits maturation of dendritic cells in autoimmune hepatitis.
Application : WB
Reactivity : Mouse
Lu CC et al. Int J Oncol 2019; 54 (4):1271-1281
Lin TK et al. Oxid Med Cell Longev 2019; 2019:9537504 Mitochondrial Transfer of Wharton's Jelly Mesenchymal Stem Cells Eliminates Mutation Burden and Rescues Mitochondrial Bioenergetics in Rotenone-Stressed MELAS Fibroblasts.
Reactivity : Human
XiaotianZhang et al. Nutrition, Metabolism and Cardiovascular Diseases 2019; (Epub) MicroRNA-217-5p ameliorates endothelial cell apoptosis induced by ox-LDL by targeting CLIC4.
Gowri Nayak et al. bioRxiv 2019; (Epub) Adaptive thermogenesis in mice requires adipocyte light-sensing via Opsin 3.
Application : IHC-Fr
Reactivity : Mouse
Wang Y et al. Acta Histochem 2019; 121 (1):56-63 Protective effects of astaxanthin on subarachnoid hemorrhage-induced early brain injury: Reduction of cerebral vasospasm and improvement of neuron survival and mitochondrial function.
Application : WB
Reactivity : Rat
Tu HJ et al. Clin Epigenetics 2018; 10 (1):162 The anticancer effects of MPT0G211, a novel HDAC6 inhibitor, combined with chemotherapeutic agents in human acute leukemia cells.
Application : WB
Reactivity : Human
Fischer C et al. Nat Commun 2017; 8 (1):2079 A miR-327-FGF10-FGFR2-mediated autocrine signaling mechanism controls white fat browning.
Application : IHC-P
Reactivity : Mouse
Muller TA et al. Biochem Biophys Res Commun 2017; (Epub) Characterization of human AlkB homolog 1 produced in mammalian cells and demonstration of mitochondrial dysfunction in ALKBH1-deficient cells.
Application : WB
Reactivity : Human
Kuo SM et al. MBio 2017; 8 (3) Inhibition of Avian Influenza A Virus Replication in Human Cells by Host Restriction Factor TUFM Is Correlated with Autophagy.
Application : WB
Reactivity : Human
Lee H et al. J Biol Chem 2017; (Epub) The Short Variant of the Mitochondrial Dynamin OPA1 Maintains Mitochondrial Energetics and Cristae Structure.
Application : WB
Reactivity : Human
Chen YH et al. Journal of Diabetes Research 2016; 2016:Article ID 1828071 the different effects of * and * on cell death and parp activity in pancreatic nit-1 cells.
Application : WB
Reactivity : Mouse
Xu TJ et al. Eur Rev Med Pharmacol Sci 2016; 20 (15):3297-303 * in combination with selenium inhibits hg/pal-induced cardiomyocyte apoptosis by cbl-b regulating p38mapk/cbp/ku70 pathway.
Application : WB
Reactivity : Rat
Shindo Y et al. Sci Rep 2016; 6:29224 An Artificial Reaction Promoter Modulates Mitochondrial Functions via Chemically Promoting Protein Acetylation.
Application : WB
Reactivity : Human
Niu Z et al. Environ Toxicol Pharmacol 2016; 45:212-221 mitophagy inhibits proliferation by decreasing cyclooxygenase-2 (cox-2) in *-treated hepg2 cells.
Application : WB
Reactivity : Human
Cho EC et al. Mediators Inflamm 2015; 2015:287345 RRM2B-Mediated Regulation of Mitochondrial Activity and Inflammation under Oxidative Stress.
Application : WB
Reactivity : Human
Ishii Y et al. PLoS One 2015; 10 (10):e0140585 Knockout Serum Replacement Promotes Cell Survival by Preventing BIM from Inducing Mitochondrial Cytochrome C Release.
Application : WB
Reactivity : Human
Yi-Wen Lo et al. Journal of Cellular and Molecular Medicine 2015; 19 (4):744-59 mitochondrial proteomics with sirna knockdown to reveal acat1 and mdh2 in the development of *-resistant uterine cancer
Application : WB
Reactivity : Human
Cho EC et al. Oncotarget 2014; 5 (13):4834-44 Tumor suppressor FOXO3 regulates ribonucleotide reductase subunit RRM2B and impacts on survival of cancer patients.
Application : WB
Reactivity : Human
Hanaoka Y et al. PLoS One 2014; 9 (4):e94930 Tissue Inhibitor of Metalloproteinase-3 Knockout Mice Exhibit Enhanced Energy Expenditure through Thermogenesis.
Application : WB
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169