Calretinin antibody

Name: Calretinin antibody
Brand: GeneTex
SKU: GTX103261
Price: 399.00 USD
Rating: 3 (1 reviews)

Anti-Calretinin antibody used in IHC (Paraffin sections) (IHC-P). GTX103261

Anti-Calretinin antibody used in Western Blot (WB). GTX103261

Anti-Calretinin antibody used in IHC (Frozen sections) (IHC-Fr). GTX103261

Anti-Calretinin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103261

Cat. No. GTX103261

Reference 5

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all Calretinin products

View product citations for antibody GTX103261 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	1:100-1:1000

Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

A431 , Mouse brain , human CALB2-transfected 293T cells , rat brain

Predict Reactivity

Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.08 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calretinin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949798

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calbindin 2 , CAB29 , CAL2 , CR

Background

This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. Three alternatively spliced transcript variants that encode different proteins have been described. [provided by RefSeq]

Database

Gene ID: 794 CALB2
UniProt: P22676 CALB2

Research Area

DATA IMAGES

GTX103261 IHC-P Image

Calretinin antibody detects Calretinin protein at cytosol on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
Calretinin antibody (GTX103261) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103261 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Mouse retina, using Calretinin(GTX103261) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103261 WB Image

Calretinin antibody detects CALB2 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human CALB2-transfected 293T cells
12% SDS-PAGE
Calretinin antibody (GTX103261) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103261 IHC-Fr Image

Calretinin antibody detects Calretinin protein by immunohistochemical analysis.
Samples: Frozen Section adult mouse hippocampus.
Green: Calretinin protein stained by Calretinin antibody (GTX103261) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX103261 ICC/IF Image

Calretinin antibody detects Calretinin protein at cytoplasm by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calretinin protein stained by Calretinin antibody (GTX103261) diluted at 1:200.
Blue: Hoechst 33342 staining.

GTX103261 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Calretinin antibody (GTX103261) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103261 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX103261 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103261 IHC-Fr Image

Calretinin antibody detects Calretinin protein in the amacrine cells by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Calretinin protein stained by Calretinin antibody (GTX103261) diluted at 1:250.
Red: Protein kinase C alpha staining.
Blue: Fluoroshield with DAPI (GTX30920).

GTX103261 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Calretinin antibody (GTX103261) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GTX103261 IHC-P Image

Calretinin antibody detects Calretinin protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Calretinin stained by Calretinin antibody (GTX103261) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Hosoya M et al. Dev Neurobiol 2021; 81 (8) : 905-938 Neuronal development in the cochlea of a nonhuman primate model, the common marmoset.

Hosoya M et al. FEBS J 2021; 288 (1) : 325-353 The common marmoset as suitable nonhuman alternative for the analysis of primate cochlear development.

Hsu WH et al. Aging (Albany NY) 2020; 12 (11) : 10556-10577 T1-11, an adenosine derivative, ameliorates aging-related behavioral physiology and senescence markers in aging mice.

Application : WB

Reactivity : Human

Vogt D et al. Curr Eye Res 2020; : 1-8 Premacular Cells as Source of Neurotrophic Factors in Idiopathic Macular Holes.

Secondo A et al. Cell Death Discovery 2018; (Epub) Na+/Ca2+ exchanger 1 on nuclear envelope controls PTEN/Akt pathway via nucleoplasmic Ca2+ regulation during neuronal differentiation.

Application : WB

Reactivity : Rat

Submit a Reference

REVIEW