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Desmoglein 2 antibody [N1N2], N-term

Orthogonal
Anti-Desmoglein 2 antibody [N1N2], N-term used in Western Blot (WB). GTX102508
Anti-Desmoglein 2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102508
Anti-Desmoglein 2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102508
Anti-Desmoglein 2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102508
Anti-Desmoglein 2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102508
Anti-Desmoglein 2 antibody [N1N2], N-term used in Western Blot (WB). GTX102508

Cat. No. GTX102508

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Desmoglein 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

122 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.36 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human Desmoglein 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950146

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

desmoglein 2 , CDHF5 , HDGC

Cellular Localization

Cell membrane , Cell junction , desmosome

Background

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. This gene product is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This second family member is expressed in colon, colon carcinoma, and other simple and stratified epithelial-derived cell lines. Mutations in this gene have been associated with arrhythmogenic right ventricular dysplasia, familial, 10. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Desmoglein 2 antibody [N1N2], N-term used in Western Blot (WB). GTX102508

GTX102508 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Desmoglein 2 antibody [N1N2], N-term (GTX102508) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Desmoglein 2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102508

GTX102508 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DU145 xenograft , using Desmoglein-2(GTX102508) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Desmoglein 2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102508

GTX102508 IHC-P Image

Desmoglein 2 antibody [N1N2], N-term detects Desmoglein 2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
Desmoglein 2 stained by Desmoglein 2 antibody [N1N2], N-term (GTX102508) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Desmoglein 2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102508

GTX102508 ICC/IF Image

Desmoglein 2 antibody [N1N2], N-term detects Desmoglein 2 protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Desmoglein 2 stained by Desmoglein 2 antibody [N1N2], N-term (GTX102508) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Desmoglein 2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102508

GTX102508 IHC-P Image

Desmoglein 2 antibody [N1N2], N-term detects Desmoglein 2 protein at cell membrane and cytoplasm in mouse skin by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
Desmoglein 2 antibody [N1N2], N-term (GTX102508) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Desmoglein 2 antibody [N1N2], N-term used in Western Blot (WB). GTX102508

GTX102508 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Desmoglein 2 antibody [N1N2], N-term (GTX102508) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Liu Y et al. Am J Physiol Gastrointest Liver Physiol 2017; 313 (5) : G478-G491 Kr?ppel-like factor 5 is essential for maintenance of barrier function in mouse colon.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169