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EasyBlocker

EasyBlocker. GTX425858
EasyBlocker. GTX425858

Cat. No. GTX425858

Reference 10

Application

 WB
Package
60 g ($249),
12 g ($99)
See all Blocking reagent products
PRODUCT

Summary

EasyBlocker for EasyBlot kit

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
Not tested in other applications.

PROPERTIES

Form

Lyophilized powder

Buffer

PBS

Preservative

No Preservative

Storage

Store at 4ºC.

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Easy Blot kit , EasyBlocker , Easy Blot , EasyBlot kit , EasyBlot

Background

EasyBlocker efficiently eliminates background signal caused by Protein A/G.

DATA IMAGES

EasyBlocker. GTX425858

GTX425858 WB Image

EasyBlocker Western blot analysis of protein G sample. Membrane was blocked with 5% milk (A and C) or EasyBlocker (B and D). A and B: 1st antibody: Rabbit IgG 2nd antibody: EasyBlot anti-Rabbit IgG HRP C and D: 1st antibody: Mouse IgG 2nd antibody: EasyBlot anti-Mouse IgG HRP

EasyBlocker. GTX425858

GTX425858 WB Image

EasyBlocker Western blot analysis of protein A sample. Membrane was blocked with (A) 5% milk or (B) EasyBlocker. Rabbit anti-GST antibody was then used as 1st antibody followed by EasyBlot anti-Rabbit IgG HRP as second step reagent.

REFERENCE

Application Reference

Laubscher D et al. Nat Commun 2021; 12 (1) : 6924 BAF complexes drive proliferation and block myogenic differentiation in fusion-positive rhabdomyosarcoma.
Gajdiss M et al. Microorganisms 2020; 8 (6) YycH and YycI Regulate Expression of Staphylococcus aureus Autolysins by Activation of WalRK Phosphorylation.
Chan H et al. Microbiologyopen 2020; : e999 Spo0J and SMC are required for normal chromosome segregation in Staphylococcus aureus.
Lin RJ et al. Cancer Manag Res 2018; 10 : 4237-4248 Capsaicin-induced TRIB3 upregulation promotes apoptosis in cancer cells.
Ian R. Monk et al. bioRxiv 2018; (Epub) Zinc-binding to the cytoplasmic PAS domain regulates the essential WalK histidine kinase of Staphylococcus aureus.
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REVIEW

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Application Reference
Laubscher D et al. Nat Commun 2021; 12 (1):6924 BAF complexes drive proliferation and block myogenic differentiation in fusion-positive rhabdomyosarcoma.
Gajdiss M et al. Microorganisms 2020; 8 (6) YycH and YycI Regulate Expression of Staphylococcus aureus Autolysins by Activation of WalRK Phosphorylation.
Chan H et al. Microbiologyopen 2020;:e999 Spo0J and SMC are required for normal chromosome segregation in Staphylococcus aureus.
Lin RJ et al. Cancer Manag Res 2018; 10:4237-4248 Capsaicin-induced TRIB3 upregulation promotes apoptosis in cancer cells.
Ian R. Monk et al. bioRxiv 2018; (Epub) Zinc-binding to the cytoplasmic PAS domain regulates the essential WalK histidine kinase of Staphylococcus aureus.
Gono T et al. Mod Rheumatol 2018;:43117 Improved quantification of a commercial enzyme-linked immunosorbent assay kit for measuring anti-MDA5 antibody.
Numata S et al. J Biol Chem 2014; 289 (12):8420-31 CvfA protein and polynucleotide phosphorylase act in an opposing manner to regulate Staphylococcus aureus virulence.
Wu MH et al. Oncogene 2014; (Epub) MCT-1 expression and PTEN deficiency synergistically promote neoplastic multinucleation through the Src/p190B signaling activation.
Kaito C et al. PLoS Pathog 2013; 9 (4):e1003269 Mobile genetic element SCCmec-encoded psm-mec RNA suppresses translation of agrA and attenuates MRSA virulence.
Huang JT et al. J Virol 2013; 87 (9):4994-5004 Hepatitis C virus replication is modulated by the interaction of nonstructural protein NS5B and fatty acid synthase.
SDS
PBS.pdf
Package List Price ($)
$ 249
$ 99