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GSK3 beta (phospho Ser9) antibody

Orthogonal
Anti-GSK3 beta (phospho Ser9) antibody used in Western Blot (WB). GTX86837
Anti-GSK3 beta (phospho Ser9) antibody used in IHC (Paraffin sections) (IHC-P). GTX86837
Anti-GSK3 beta (phospho Ser9) antibody used in Western Blot (WB). GTX86837
Anti-GSK3 beta (phospho Ser9) antibody used in Western Blot (WB). GTX86837

Cat. No. GTX86837

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all GSK3 beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
IHC-P 1:50~1:100
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human GSK3 beta (1-50).

Purification

Purified by antigen-affinity chromatography. Non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
From serum

Conjugation

Unconjugated

RRID

AB_10724042

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

GSK3B , glycogen synthase kinase 3 beta , GSK3 beta

Cellular Localization

Cytoplasm,Nucleus,Cell membrane

Background

The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-GSK3 beta (phospho Ser9) antibody used in Western Blot (WB). GTX86837

GTX86837 WB Image

WB analysis of HeLa cells treated with EGF lysate using GTX86837 GSK3 beta (phospho Ser9) antibody. The lane on the right is blocked with the synthesized peptide.

Anti-GSK3 beta (phospho Ser9) antibody used in IHC (Paraffin sections) (IHC-P). GTX86837

GTX86837 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX86837 GSK3 beta (phospho Ser9) antibody. The picture on the right is blocked with the synthesized peptide.

Anti-GSK3 beta (phospho Ser9) antibody used in Western Blot (WB). GTX86837

GTX86837 WB Image

Orthogonal

The data was published in the 2022 in Int J Mol Sci. PMID: 35563135

Anti-GSK3 beta (phospho Ser9) antibody used in Western Blot (WB). GTX86837

GTX86837 WB Image

The data was published in the 2022 in Int J Mol Sci. PMID: 35563135

REFERENCE

Application Reference

Anna Mietelska-Porowska et al. Int J Mol Sci 2022; 23 (9) Induction of Brain Insulin Resistance and Alzheimer's Molecular Changes by Western Diet.
Application : WB
Reactivity : Mouse
Lin HC et al. J Agric Food Chem 2017; 65 (1) : 51-59 Antroquinonol, a Ubiquinone Derivative from the Mushroom Antrodia camphorata, Inhibits Colon Cancer Stem Cell-like Properties: Insights into the Molecular Mechanism and Inhibitory Targets.
Application : WB
Reactivity : Human
Kuo YH et al. BMC Complement Altern Med 2017; 17 (1) : 71 N-(4-methoxyphenyl) caffeamide-induced melanogenesis inhibition mechanisms.
Application : WB
Reactivity : Mouse
Chen MJ et al. Sci Rep 2016; 6 : 34933 PAK1 confers chemoresistance and poor outcome in non-small cell lung cancer via β-catenin-mediated stemness.
Application : WB
Reactivity : Human
Wu JY et al. Evid Based Complement Alternat Med 2013; 2013 : 579751 Anti-Bladder-Tumor Effect of Baicalein from Scutellaria baicalensis Georgi and Its Application In Vivo.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399