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MUC1 antibody

Anti-MUC1 antibody used in Western Blot (WB). GTX100459
Anti-MUC1 antibody used in Immunoprecipitation (IP). GTX100459
Anti-MUC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100459
Anti-MUC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100459
Anti-MUC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100459
Anti-MUC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100459

Cat. No. GTX100459

Reference 2
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MUC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

122 kDa. ( Note )

Positive Control

MCF-7

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human MUC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618561

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mucin 1, cell surface associated , ADMCKD , ADMCKD1 , CA 15-3 , CD227 , EMA , H23AG , KL-6 , MAM6 , MCD , MCKD , MCKD1 , MUC-1 , MUC-1/SEC , MUC-1/X , MUC1/ZD , PEM , PEMT , PUM

Cellular Localization

Apical cell membrane; Single-pass type I membrane protein , Isoform 5: Secreted , Isoform 7: Secreted , Isoform 9: Secreted , Mucin-1 subunit beta: Cell membrane , Cytoplasm , Nucleus

Background

This gene is a member of the mucin family and encodes a membrane bound, glycosylated phosphoprotein. The protein is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. It also includes a 20 aa variable number tandem repeat (VNTR) domain, with the number of repeats varying from 20 to 120 in different individuals. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length nature of only some has been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MUC1 antibody used in Western Blot (WB). GTX100459

GTX100459 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MUC1 antibody (GTX100459) diluted at 1:1000.
The observed M.W. is based on the publication: PMID: 21927028

Anti-MUC1 antibody used in Immunoprecipitation (IP). GTX100459

GTX100459 IP Image

Immunoprecipitation of MUC1 protein from HCT-116 whole cell extracts using 5 μg of MUC1 antibody (GTX100459).
Western blot analysis was performed using MUC1 antibody (GTX100459) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-MUC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100459

GTX100459 ICC/IF Image

MUC1 antibody detects MUC1 protein at cytoplasm by immunofluorescent analysis.
Sample: HCT116 cells were fixed in ice-cold MeOH for 5 min.
Green: MUC1 protein stained by MUC1 antibody (GTX100459) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-MUC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100459

GTX100459 IHC-P Image

MUC1 antibody detects MUC1 protein at cytoplasm by immunohistochemical analysis (Autostainer Formulated).
Sample: Paraffin-embedded human breast cancer.
MUC1 stained by MUC1 antibody (GTX100459) diluted at 1:500.
Antigen Retrieval: Citrate buffer, 20 min

Anti-MUC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100459

GTX100459 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using MUC1(GTX100459) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

Anti-MUC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100459

GTX100459 IHC-P Image

MUC1 antibody detects MUC1 protein at cytoplasm in human cervical cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical cancer.
MUC1 antibody (GTX100459) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Chang YX et al. Cancers (Basel) 2020; 12 (12) Chaperonin-Containing TCP-1 Promotes Cancer Chemoresistance and Metastasis through the AKT-GSK3β-β-Catenin and XIAP-Survivin Pathways.
Application : WB
Reactivity : Human
Souris JS et al. Carcinogenesis 2019; A Novel Mouse Model of Sporadic Colon Cancer Induced by Combination of Conditional Apc Genes and Chemical Carcinogen in the Absence of Cre Recombinase.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

MUC1 antibody Cat. No. GTX100459

Rating
( Average 5 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-MUC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100459
Date : Anonymous submitted on 21-Oct-2020
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : breast cancer tissue
Fixation : Citrate buffer, 0.3°C
Primary Antibody : 1:500, 4°C, 16Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169