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Musashi 2 antibody [N1C1]

Anti-Musashi 2 antibody [N1C1] used in Western Blot (WB). GTX117808
Anti-Musashi 2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX117808
Anti-Musashi 2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX117808
Anti-Musashi 2 antibody [N1C1] used in IHC (Frozen sections) (IHC-Fr). GTX117808
Anti-Musashi 2 antibody [N1C1] used in Western Blot (WB). GTX117808
Anti-Musashi 2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX117808

Cat. No. GTX117808

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

MCF-7 , Mouse brain

Product Note

Knockdown/Knockout validation was supported by references (PMID:26990993)

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Musashi 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10722801

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

musashi RNA binding protein 2 , MSI2H

Cellular Localization

Cytoplasm

Background

This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Musashi 2 antibody [N1C1] used in Western Blot (WB). GTX117808

GTX117808 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX117808 diluted at 1:1000

Anti-Musashi 2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX117808

GTX117808 IHC-P Image

Musashi 2 antibody [N1C1] detects Musashi 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Musashi 2 stained by Musashi 2 antibody [N1C1] (GTX117808) diluted at 1:500.
Antigen Retrieval: Trilogy™M (EDTA based) buffer, 15min

Anti-Musashi 2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX117808

GTX117808 IHC-P Image

Musashi 2 antibody [N1C1] detects Musashi 2 protein at cytosol on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Musashi 2 antibody [N1C1] (GTX117808) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Musashi 2 antibody [N1C1] used in IHC (Frozen sections) (IHC-Fr). GTX117808

GTX117808 IHC-Fr Image

Musashi 2 antibody [N1C1] detects Musashi 2 protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Musashi 2 protein stained by Musashi 2 antibody [N1C1] (GTX117808) diluted at 1:250.
Red: Protein kinase C alpha staining.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Musashi 2 antibody [N1C1] used in Western Blot (WB). GTX117808

GTX117808 WB Image

Sample (30 ug of whole cell lysate)
A: MCF-7
10% SDS PAGE
GTX117808 diluted at 1:1000

Anti-Musashi 2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX117808

GTX117808 IHC-P Image

Musashi 2 antibody [N1C1] detects Musashi 2 protein at cytosol on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
Musashi 2 antibody [N1C1] (GTX117808) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

Musashi 2 antibody [N1C1] Cat. No. GTX117808

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 07-Mar-2019
Score :
Application Tested : WB
Sample Species : Hu
Sample : K562 cells
Amount used : 1.2 x 10^4 cells/well
Blocking : 1% skim milk, 20°C, 1Hr
Primary Antibody : 1:1000, 10°C, 12Hr
Lane Description : Lane 1: Control siRNA, Lane 2: MSI2 siRNA
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169