NFkB p65 (phospho Thr435) antibody

Cat. No. GTX78994

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Monkey
Package	100 μg ($399)

See all NFkB p65 products

Datasheet

View product citations for antibody GTX78994 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500 - 1:1000
IHC-P	1:50 - 1:100

Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human NF-κB around the phosphorylation site of threonine 435 (E-G-Tp-L-S).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11168681

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NFKB3,RELA,RELA protooncogene, NFkB subunit,p65,NFkB p65,NF-κb p65,RELA proto-oncogene, NF-kB subunit

Cellular Localization

Nucleus,Cytoplasm

Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Database

Gene ID: 5970 RELA
UniProt: Q04206 RELA

Research Area

DATA IMAGES

GTX78994 WB Image

WB analysis of TNF alpha treated COS7 cell lysate using GTX78994 NFkB p65 (phospho Thr435) antibody (Lane 3 and 4) and NFkB p65 antibody (Lane 1 and 2).

GTX78994 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX78994 NFkB p65 (phospho Thr435) antibody.

REFERENCE

There are currently no references for NFkB p65 (phospho Thr435) antibody (GTX78994). Be the first to share your publications with this product.

Submit a Reference

REVIEW

There are currently no reviews for NFkB p65 (phospho Thr435) antibody (GTX78994). Be the first to share your experience with this product.

Submit a Review

Package

List Price ($)

100 μg

$ 399

Qty