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Src (phospho Tyr527) antibody

Orthogonal
Anti-Src (phospho Tyr527) antibody used in IHC (Paraffin sections) (IHC-P). GTX50210
Anti-Src (phospho Tyr527) antibody used in Western Blot (WB). GTX50210
Anti-Src (phospho Tyr527) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50210
Anti-Src (phospho Tyr527) antibody used in Western Blot (WB). GTX50210
Anti-Src (phospho Tyr527) antibody used in Western Blot (WB). GTX50210

Cat. No. GTX50210

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all Src products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Product Note

Based on sequence homology, this antibody may cross with other Src family members such as YES1 , FYN proteins when phosphorylated at the equivalent sites.

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 529 (P-Q-Y(p)-Q-P) derived from human Src. The Tyr529 of human Src is equivalent to Tyr527 of chicken Src.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

RRID

AB_11171453

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SRC proto-oncogene, non-receptor tyrosine kinase , ASV , SRC1 , THC6 , c-SRC , p60-Src

Cellular Localization

Cell membrane,Mitochondrion inner membrane,Nucleus,Cytoplasm, cytoskeleton

Background

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Src (phospho Tyr527) antibody used in IHC (Paraffin sections) (IHC-P). GTX50210

GTX50210 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50210 Src (phospho Tyr527) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-Src (phospho Tyr527) antibody used in Western Blot (WB). GTX50210

GTX50210 WB Image

WB analysis of extracts from HT-29 cells untreated or treated with PMA using GTX50210 Src (phospho Tyr527) antibody.

Anti-Src (phospho Tyr527) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50210

GTX50210 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50210 Src (phospho Tyr527) antibody.

Anti-Src (phospho Tyr527) antibody used in Western Blot (WB). GTX50210

GTX50210 WB Image

WB analysis of extracts from HepG2 cells treated with Anisomycin or calf intestinal phosphatase (CIP) using GTX50210 Src (phospho Tyr527) antibody.

Anti-Src (phospho Tyr527) antibody used in Western Blot (WB). GTX50210

GTX50210 WB Image

Orthogonal

The data was published in the journal Evid Based Complement Alternat Med in 2012.PMID: 23320038

REFERENCE

Application Reference

Hsu HY et al. Evid Based Complement Alternat Med 2012; 2012 : 819632 Antimigratory Effects of the Methanol Extract from Momordica charantia on Human Lung Adenocarcinoma CL1 Cells.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399