GeneTex

  • Country / Location Selection

United States (US)

Trident Prestained Protein Ladder (High Range)

Trident Prestained Protein Ladder (High Range). GTX50875
Trident Prestained Protein Ladder (High Range). GTX50875

Cat. No. GTX50875

Reference 15

Application

 WB
Package
500 μl ($129)
See all Protein Ladder products
PRODUCT

Summary

The Prestained Protein Ladder is a three-color protein standard with 12 pre-stained proteins covering a wide range molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use.

APPLICATION

Application Note

Under suggested conditions, Prestained Protein Ladder resolves 12 major bands in 15% SDS-PAGE (Trisglycine buffer) and after Western blotting to nitrocellulose membrane.

PROPERTIES

Form

Liquid

Buffer

20mM Tris-Phosphate pH7.5 at 25ºC), 2 % SDS, 1mM Dithiothreitol, 3.6M Urea, 15 % (v/v) Glycerol

Preservative

No preservative

Storage

Store at 4ºC short term (3 months). For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

Trident Prestained Protein Ladder (High Range). GTX50875

GTX50875 Image

Migration pattern of GTX50875 Trident Prestained Protein Ladder (High Range) in Tris-Glycine gel.

Trident Prestained Protein Ladder (High Range). GTX50875

GTX50875 Image

Migration pattern of GTX50875 Trident Prestained Protein Ladder (High Range) in Bis-Tris- gel.

REFERENCE

Application Reference

Santos MF et al. Nat Commun 2023; 14 (1) : 4588 HIV-1-induced nuclear invaginations mediated by VAP-A, ORP3, and Rab7 complex explain infection of activated T cells.
Serrano-Rivero Y et al. Molecules 2023; 28 (10) Selecting Nanobodies Specific for the Epidermal Growth Factor from a Synthetic Nanobody Library.
S Park et al. J Bacteriol 2023; : e0000323 Manganese Acts as an Environmental Inhibitor of Pseudomonas aeruginosa Biofilm Development by Inducing Dispersion and Modulating c-di-GMP and Exopolysaccharide Production via RbdA
Beesley S et al. J Neurophysiol 2022; 127 (6) : 1496-1510 GluN3 subunit expression correlates with increased vulnerability of hippocampus and entorhinal cortex to neurodegeneration in a model of temporal lobe epilepsy.
Zhang DY et al. ACS Chem Neurosci 2022; 13 (13) : 1979-1991 Monosialotetrahexosylganglioside Promotes Early Aβ42 Oligomer Formation and Maintenance.
Show More +
Submit a Reference

REVIEW

There are currently no reviews for Trident Prestained Protein Ladder (High Range) (GTX50875). Be the first to share your experience with this product.
Submit a Review
Application Reference
Santos MF et al. Nat Commun 2023; 14 (1):4588 HIV-1-induced nuclear invaginations mediated by VAP-A, ORP3, and Rab7 complex explain infection of activated T cells.
Serrano-Rivero Y et al. Molecules 2023; 28 (10) Selecting Nanobodies Specific for the Epidermal Growth Factor from a Synthetic Nanobody Library.
S Park et al. J Bacteriol 2023;:e0000323 Manganese Acts as an Environmental Inhibitor of Pseudomonas aeruginosa Biofilm Development by Inducing Dispersion and Modulating c-di-GMP and Exopolysaccharide Production via RbdA
Beesley S et al. J Neurophysiol 2022; 127 (6):1496-1510 GluN3 subunit expression correlates with increased vulnerability of hippocampus and entorhinal cortex to neurodegeneration in a model of temporal lobe epilepsy.
Zhang DY et al. ACS Chem Neurosci 2022; 13 (13):1979-1991 Monosialotetrahexosylganglioside Promotes Early Aβ42 Oligomer Formation and Maintenance.
Santos MF et al. Cells 2022; 11 (16) Anti-Human CD9 Fab Fragment Antibody Blocks the Extracellular Vesicle-Mediated Increase in Malignancy of Colon Cancer Cells.
Santos MF et al. J Extracell Vesicles 2021; 10 (10):e12132 Itraconazole inhibits nuclear delivery of extracellular vesicle cargo by disrupting the entry of late endosomes into the nucleoplasmic reticulum.
Shubin CB et al. Elife 2020; 9 The role of Rif1 in telomere length regulation is separable from its role in origin firing.
Valle-Bautista R et al. Front Cell Dev Biol 2020; 8:564561 Impaired Cortical Cytoarchitecture and Reduced Excitability of Deep-Layer Neurons in the Offspring of Diabetic Rats.
M?rquez-Valadez B et al. Front Neurosci 2019; 13:360 The Systemic Administration of the Histamine H1 Receptor Antagonist/Inverse Agonist Chlorpheniramine to Pregnant Rats Impairs the Development of Nigro-Striatal Dopaminergic Neurons.
Berenice M?rquez-Valadez et al. Front. Neurosci. 2019; (Epub) The Systemic Administration of the Histamine H1 Receptor Antagonist/Inverse Agonist.
Khandelwal S et al. Int J Mol Sci 2018; 19 (11) Cytotoxicity of Selenium Immunoconjugates against Triple Negative Breast Cancer Cells.
Santos MF et al. J Biol Chem 2018; (Epub) VAMP-associated protein-A and oxysterol-binding protein-related protein 3 promote the entry of late endosomes into the nucleoplasmic reticulum.
Rappa G et al. Oncotarget 2015; 6 (10):7970-91 Tetraspanin CD9 determines invasiveness and tumorigenicity of human breast cancer cells.
Rappa G et al. Mol Cancer Res 2014; The Nuclear Pool of Tetraspanin CD9 Contributes to Mitotic Processes in Human Breast Carcinoma.
SDS
Glycerol.pdf
Package List Price ($)
$ 129