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EasyBlot, finally a solution to masking by denatured IgG!

Immunoprecipitation (IP) and co-Immunoprecipitation (co-IP) assays often necessitate the use of the same, or same-host, antibodies for both the IP and western blot steps of these procedures. In these situations the target protein signal can be partially or completely masked on the western blot by secondary antibody detection of the denatured heavy and light chains of the IP antibody. GeneTex’s collection of EasyBlot secondary antibodies eliminates this problem by binding only to the non-denatured primary antibody used for the western blot while ignoring the denatured IgG chains of the IP antibody. In addition, GeneTex’s EasyBlocker reagent eliminates background signal caused by free Protein A or Protein G. Together, these reagents will help you to get the cleanest results on your IP/co-IP experiments.

 

 

 


 

EasyBlot: the easiest way to get the best result.

 

Clear   Efficiently eliminates denatured IgG masking of your target protein’s signal.
Compatible   Works well for immunoprecipitations and co-immunoprecipitations using Protein A-, Protein G-, or Agarose-conjugated antibodies.
Comprehensive   Individual EasyBlot reagents to detect primary antibodies from rabbit, mouse, rat, goat or sheep hosts.
Convenient   Simple! Just replace your regular secondary antibody with the appropriate EasyBlot secondary antibody! For background caused by free Protein A or Protein G, try our EasyBlocker reagent available alone or in a convenient kit form combined with individual EasyBlot secondary antibodies.


 



Eliminate Cat. No. Product Package
Masking bands by denatured IgG GTX221666-01 EasyBlot anti-Rabbit IgG (HRP) 50 μl 250 μl
GTX221667-01 EasyBlot anti-Mouse IgG (HRP)
GTX628474-01 EasyBlot anti-Rat IgG (HRP)
GTX628547-01 EasyBlot anti-Goat IgG (HRP)
GTX628906-01 EasyBlot anti-Sheep IgG (HRP)
Masking bands by denatured IgG & background from protein A/G GTX225856-01 EasyBlot anti-Rabbit IgG Kit (w/ EasyBlocker)
GTX225857-01 EasyBlot anti-Mouse IgG Kit (w/ EasyBlocker)
GTX228909-01 EasyBlot anti-Rat IgG Kit (w/ EasyBlocker)
GTX228910-01 EasyBlot anti-Goat IgG Kit (w/ EasyBlocker)
GTX228911-01 EasyBlot anti-Sheep IgG Kit (w/ EasyBlocker)
Background from protein A/G GTX425858 EasyBlocker 12 g 62 g

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All products are for research use only—Not for use in diagnostic procedures.