EasyBlot, finally a solution to masking by denatured IgG!
| Immunoprecipitation (IP) and co-Immunoprecipitation (co-IP) assays often necessitate the use of the same, or same-host, antibodies for both the IP and western blot steps of these procedures. In these situations the target protein signal can be partially or completely masked on the western blot by secondary antibody detection of the denatured heavy and light chains of the IP antibody. GeneTex’s collection of EasyBlot secondary antibodies eliminates this problem by binding only to the non-denatured primary antibody used for the western blot while ignoring the denatured IgG chains of the IP antibody. In addition, GeneTex’s EasyBlocker reagent eliminates background signal caused by free Protein A or Protein G. Together, these reagents will help you to get the cleanest results on your IP/co-IP experiments. |
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EasyBlot: the easiest way to get the best result.
| Clear |
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Efficiently eliminates denatured IgG masking of your target protein’s signal. |
| Compatible |
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Works well for immunoprecipitations and co-immunoprecipitations using Protein A-, Protein G-, or Agarose-conjugated antibodies. |
| Comprehensive |
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Individual EasyBlot reagents to detect primary antibodies from rabbit, mouse, rat, goat or sheep hosts. |
| Convenient |
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Simple! Just replace your regular secondary antibody with the appropriate EasyBlot secondary antibody! For background caused by free Protein A or Protein G, try our EasyBlocker reagent available alone or in a convenient kit form combined with individual EasyBlot secondary antibodies. |
