GeneTex

  • Country / Location Selection

United States (US)

p53 antibody

Orthogonal
Anti-p53 antibody used in Western Blot (WB). GTX128135
Anti-p53 antibody used in Western Blot (WB). GTX128135

Cat. No. GTX128135

Reference 25
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish p53 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

3dpf zebrafish embryos treated with UVC

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.47 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of zebrafish p53. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2864277

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tumor protein p53 , brp53 , drp53 , etID22686.5 , fb40d06 , p53 , wu:fb40d06 , zgc:111919

Cellular Localization

Cytoplasm , Nucleus

Background

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of bax and fas antigen expression, or by repression of Bcl-2 expression.

Database

Research Area

DATA IMAGES

Anti-p53 antibody used in Western Blot (WB). GTX128135

GTX128135 WB Image

Orthogonal

Untreated (–) and treated (+) Zebrafish embryos extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody (GTX128135) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p53 antibody used in Western Blot (WB). GTX128135

GTX128135 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2015.PMID: 26507500

REFERENCE

Application Reference

Varineau JE et al. Dis Model Mech 2024; 17 (2) A common cellular response to broad splicing perturbations is characterized by metabolic transcript downregulation driven by the Mdm2-p53 axis.
CW Cheng et al. Cancers (Basel) 2024; 16 (3) : 657 miR-622 Increases miR-30a Expression through Inhibition of Hypoxia-Inducible Factor 1α to Improve Metastasis and Chemoresistance in Human Invasive Breast Cancer Cells
Application : WB
Sun K et al. iScience 2023; 26 (11) : 108103 The splicing factor DHX38 enables retinal development through safeguarding genome integrity.
Chen J et al. MedComm (2020) 2023; 4 (5) : e352 Identification of the natural chalcone glycoside hydroxysafflor yellow A as a suppressor of P53 overactivation-associated hematopoietic defects.
Parija M et al. Oncotarget 2023; 14 : 466-480 G-protein-coupled receptor 141 mediates breast cancer proliferation and metastasis by regulating oncogenic mediators and the p-mTOR/p53 axis.
Show More +
Submit a Reference

REVIEW

p53 antibody Cat. No. GTX128135

Rating
( Average 5 based on 2 users reviews)
Application
Anti-p53 antibody. GTX128135
Date : Anonymous submitted on 25-Dec-2017
Score :
Sample Species : Zfsh
Sample : Eye tumor tissue
Blocking : Normal goat serum, 0.5Hr
Permeabilization /Antigen retrieval : EDTA pH 9.0
Primary Antibody : 1:700, 4°C, 16Hr
Figure Note : Zebrafish p53 mutant eye tumor, p53 nuclear staining, magnification 200x (A) and 400x (B).
Anti-p53 antibody. GTX128135
Date : Anonymous submitted on 25-Dec-2017
Score :
Sample Species : Zfsh
Sample : Kidney tissue
Blocking : Normal goat serum, 0.5Hr
Permeabilization /Antigen retrieval : EDTA pH 9.0
Primary Antibody : 1:700, 4°C, 16Hr
Figure Note : Zebrafish p53 mutant kidney, positive for nuclear staining, magnification 40x (A) and 400x (B).
Submit a Review
Application Reference
Varineau JE et al. Dis Model Mech 2024; 17 (2) A common cellular response to broad splicing perturbations is characterized by metabolic transcript downregulation driven by the Mdm2-p53 axis.
CW Cheng et al. Cancers (Basel) 2024; 16 (3):657 miR-622 Increases miR-30a Expression through Inhibition of Hypoxia-Inducible Factor 1α to Improve Metastasis and Chemoresistance in Human Invasive Breast Cancer Cells
Application : WB
Sun K et al. iScience 2023; 26 (11):108103 The splicing factor DHX38 enables retinal development through safeguarding genome integrity.
Chen J et al. MedComm (2020) 2023; 4 (5):e352 Identification of the natural chalcone glycoside hydroxysafflor yellow A as a suppressor of P53 overactivation-associated hematopoietic defects.
Parija M et al. Oncotarget 2023; 14:466-480 G-protein-coupled receptor 141 mediates breast cancer proliferation and metastasis by regulating oncogenic mediators and the p-mTOR/p53 axis.
Babu S et al. Elife 2022; 11 Banp regulates DNA damage response and chromosome segregation during the cell cycle in zebrafish retina.
Application : WB
Reactivity : Zebrafish
Jingzhen Li et al. Nucleic Acids Res 2021; 49 (4):2027-2043 Prpf31 is essential for the survival and differentiation of retinal progenitor cells by modulating alternative splicing
Application : WB
Reactivity : Zebrafish
Shoffner A et al. Cell Rep 2020; 32 (9):108089 Tp53 Suppression Promotes Cardiomyocyte Proliferation during Zebrafish Heart Regeneration.
Espanola SG et al. Nat Cell Biol 2020; 22 (12):1411-1422 Haematopoietic stem cell-dependent Notch transcription is mediated by p53 through the Histone chaperone Supt16h.
Application : WB
Reactivity : Zebrafish
Mohapatra S et al. Cell Death Differ 2020; (Epub) Estrogen and estrogen receptors chauffeur the sex-biased autophagic action in liver.
Application : WB
Lu LF et al. Fish Shellfish Immunol 2020; 102:449-459 Ca2+ plays an antiviral role by increasing p53 expression to achieve protection against spring viraemia of carp virus infection.
Application : WB
Reactivity : Zebrafish
Mawed SA et al. Int J Mol Sci 2020; 21 (4) Strategy of Hepatic Metabolic Defects Induced by beclin1 Heterozygosity in Adult Zebrafish.
Application : WB
Reactivity : Zebrafish
Adriana Petrovici et al. J. Mar. Sci. Eng. 2020; 8 (2):73 Toxicity of Deltamethrin to Zebrafish Gonads Revealed by Cellular Biomarkers.
Reactivity : Zebrafish
Caihong Wang et al. Chemosphere 2020; 241 Triclosan-induced liver and brain injury in zebrafish (Danio rerio) via abnormal expression of miR-125 regulated by PKCα/Nrf2/p53 signaling pathways.
Reactivity : Zebrafish
D'Agati G et al. Dis Model Mech 2019; 12 (7) Active receptor tyrosine kinases
Li H et al. J Cell Physiol 2019; (Epub) Mitochondrial proteomics reveals the mechanism of spermatogenic cells apoptosis induced by carbon ion radiation in zebrafish.
Application : WB
Reactivity : Zebrafish
Liu PH et al. Nat Cell Biol 2019; 21 (2):203-213 An IRAK1-PIN1 signalling axis drives intrinsic tumour resistance to radiation therapy.
Application : WB
Reactivity : Zebrafish
Si J et al. Toxicology 2018; (Epub) Effects of ionizing radiation and HLY78 on the zebrafish embryonic developmental toxicity.
Application : WB
Reactivity : Zebrafish
Shtraizent N et al. Elife 2017; 6 MPI depletion enhances O-GlcNAcylation of p53 and suppresses the Warburg effect.
Application : WB
Reactivity : Zebrafish
Zhou R et al. Mutat Res 2016; 793-794:41-50 Effects of Ru(CO)3Cl-glycinate on the developmental toxicities induced by X-ray and carbon-ion irradiation in zebrafish embryos.
Application : WB
Reactivity : Zebrafish
Wei Y et al. Development 2016; (Epub) RNA polymerase III component Rpc9 regulates hematopoietic stem and progenitor cell maintenance in zebrafish.
Application : WB
Reactivity : Zebrafish
Zhang S et al. Invest Ophthalmol Vis Sci 2016; 57 (4):1991-2000 Antiviral Drug * Is a Potent Inhibitor of the Proliferation of Mller Glia-Derived Progenitors During Zebrafish Retinal Regeneration.
Application : WB
Reactivity : Zebrafish
Jing J et al. Sci Rep 2015; 5:15906 A feedback regulatory loop involving p53/miR-200 and * endocrine axis controls embryo size of zebrafish.
Application : WB
Reactivity : Zebrafish
Percival SM et al. Dis Model Mech 2015; 8 (8):941-55 Variations in dysfunction of sister chromatid cohesion in esco2 mutant zebrafish reflect the phenotypic diversity of Roberts syndrome.
Application : WB
Reactivity : Zebrafish
Tsai M et al. Dev Neurobiol 2014; 75 (9):908-26 Modulation of p53 and met expression by kr-ppel-like factor 8 regulates zebrafish cerebellar development.
Application : WB
Reactivity : Zebrafish
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169