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p53 antibody [N1], N-term

Orthogonal
Anti-p53 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100629
Anti-p53 antibody [N1], N-term used in Immunoprecipitation (IP). GTX100629
Anti-p53 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100629
Anti-p53 antibody [N1], N-term used in Western Blot (WB). GTX100629
Anti-p53 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100629
Anti-p53 antibody [N1], N-term used in Western Blot (WB). GTX100629
Anti-p53 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100629

Cat. No. GTX100629

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all p53 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human p53. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037587

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53

Cellular Localization

Cytoplasm , Nucleus , Endoplasmic reticulum

Background

This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-p53 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100629

GTX100629 ICC/IF Image

p53 antibody [N1], N-term detects p53 protein at nucleus by immunofluorescent analysis.
Samples: HCT 116 cells mock (left) and treated with 30 μM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p53 protein stained by p53 antibody [N1], N-term (GTX100629) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-p53 antibody [N1], N-term used in Immunoprecipitation (IP). GTX100629

GTX100629 IP Image

Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2.5 ug of anti-p53 antibody (GTX100629). (A) Input, 20ug of HCT116 lysates. The precipitated protein was detected by GTX100629 diluted at 1:10000. EasyBlot anti-Rabbit IgG Kit (GTX225856-01) was used in Western blot.

Anti-p53 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100629

GTX100629 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using p53(GTX100629) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-p53 antibody [N1], N-term used in Western Blot (WB). GTX100629

GTX100629 WB Image

Whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [N1], N-term (GTX100629) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p53 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100629

GTX100629 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using p53(GTX100629) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-p53 antibody [N1], N-term used in Western Blot (WB). GTX100629

GTX100629 WB Image

Orthogonal

The data was published in the journal EMBO J in 2018. PMID: 29084722

Anti-p53 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100629

GTX100629 IHC-P Image

The data was published in the 2022 in Toxics. PMID: 35622687

REFERENCE

Application Reference

Alhanouf A Aljohani et al. Toxics 2022; 10 (5) The Anti-Rheumatic Drug, Leflunomide, Induces Nephrotoxicity in Mice via Upregulation of TGFβ-Mediated p53/Smad2/3 Signaling.
Application : IHC-P
Reactivity : Mouse
Doddapaneni R et al. Oncotarget 2019; 10 (4) : 480-493 Fibroblast growth factor receptor 1 (FGFR1) as a therapeutic target in adenoid cystic carcinoma of the lacrimal gland.
Application : IHC-P
Reactivity : Human
Mlcochova P et al. EMBO J 2017; (Epub) DNA damage induced by topoisomerase inhibitors activates SAMHD1 and blocks HIV-1 infection of macrophages.
Application : WB
Reactivity : Human
Abdi J et al. Blood Cancer J 2013; 3 : e119 stimulation of toll-like receptor-1/2 combined with * increases cytotoxicity to human multiple myeloma cells.
Application : WB
Reactivity : Human
Chen YJ et al. Life Sci 2013; 92 (22) : 1081-92 The synthetic flavonoid WYC02-9 inhibits colorectal cancer cell growth through ROS-mediated activation of MAPK14 pathway.
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169