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Myeloperoxidase antibody [2C7]

Anti-Myeloperoxidase antibody [2C7] used in Flow cytometry (FACS). GTX75318
Anti-Myeloperoxidase antibody [2C7] used in IHC (Paraffin sections) (IHC-P). GTX75318

Cat. No. GTX75318

Reference 7

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2C7

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, ELISA, IHC

Reactivity

 Human, Mouse
Package
40 μg ($169)
See all Myeloperoxidase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1/500-1/1,000
IHC-Fr 1/1,000-1/5,000
FACS 1/50-1/100
ELISA Assay dependent
IHC Assay dependent

Note :

FACS
Membrane permeabilisation is required for this application.
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

Bone marrow

Product Note

GTX75318 recognises native MPO in Western blots, and the heavy chain following boiling of the sample. The antibody also recognises recombinant MPO in Western blots and weakly in ELISA. The antibody may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry following cell permeablisation.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human myeloperoxidase.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_376720

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myeloperoxidase

Cellular Localization

Lysosome

Background

Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014]

Database

Research Area

DATA IMAGES

Anti-Myeloperoxidase antibody [2C7] used in Flow cytometry (FACS). GTX75318

GTX75318 FACS Image

FACS analysis of human peripheral blood granulocytes using GTX75318 Myeloperoxidase antibody [2C7].

Anti-Myeloperoxidase antibody [2C7] used in IHC (Paraffin sections) (IHC-P). GTX75318

GTX75318 IHC-P Image

IHC-P analysis of human bone marrow tissue using GTX75318 Myeloperoxidase antibody [2C7].

REFERENCE

Application Reference

Su M et al. Nat Cardiovasc Res 2022; 1 (8) : 732-747 Gasdermin D-dependent platelet pyroptosis exacerbates NET formation and inflammation in severe sepsis.
Application : ICC/IF
Reactivity : Human
Kanakaraju Kaliannan et al. Int J Mol Sci 2022; 23 (10) Decreased Tissue Omega-6/Omega-3 Fatty Acid Ratio Prevents Chemotherapy-Induced Gastrointestinal Toxicity Associated with Alterations of Gut Microbiome.
Application : IHC-P
Reactivity : Mouse
Rocha JD et al. Sci Rep 2015; 5 : 8008 Capsular polysaccharides from Cryptococcus neoformans modulate production of neutrophil extracellular traps (NETs) by human neutrophils.
Application : ICC/IF
Reactivity : Human

Clone Reference

Hasegawa J et al. Intern Med 2018; (Epub) An Autopsy Case of Myeloperoxidase-anti-neutrophil Cytoplasmic Antibody (MPO-ANCA) -associated Vasculitis Accompanied by Cryoglobulinemic Vasculitis Affecting the Kidneys, Skin, and Gastrointestinal Tract.
Futamata E et al. Nephron 2017; (Epub) Vanishing Immunoglobulins: The Formation of Pauci-Immune Lesions in Myeloperoxidase-Antineutrophil Cytoplasmic Antibody-Associated Vasculitis.
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Application Reference
Su M et al. Nat Cardiovasc Res 2022; 1 (8):732-747 Gasdermin D-dependent platelet pyroptosis exacerbates NET formation and inflammation in severe sepsis.
Application : ICC/IF
Reactivity : Human
Kanakaraju Kaliannan et al. Int J Mol Sci 2022; 23 (10) Decreased Tissue Omega-6/Omega-3 Fatty Acid Ratio Prevents Chemotherapy-Induced Gastrointestinal Toxicity Associated with Alterations of Gut Microbiome.
Application : IHC-P
Reactivity : Mouse
Rocha JD et al. Sci Rep 2015; 5:8008 Capsular polysaccharides from Cryptococcus neoformans modulate production of neutrophil extracellular traps (NETs) by human neutrophils.
Application : ICC/IF
Reactivity : Human
Gardet A et al. J Immunol 2010; 185 (9):5577-85 LRRK2 is involved in the IFN-gamma response and host response to pathogens.
Application : IHC
Reactivity : Human
Clone Reference
Hasegawa J et al. Intern Med 2018; (Epub) An Autopsy Case of Myeloperoxidase-anti-neutrophil Cytoplasmic Antibody (MPO-ANCA) -associated Vasculitis Accompanied by Cryoglobulinemic Vasculitis Affecting the Kidneys, Skin, and Gastrointestinal Tract.
Futamata E et al. Nephron 2017; (Epub) Vanishing Immunoglobulins: The Formation of Pauci-Immune Lesions in Myeloperoxidase-Antineutrophil Cytoplasmic Antibody-Associated Vasculitis.
Brzezinska AA et al. Immunology 2009; 127 (3):386-97 Signalling mechanisms for Toll-like receptor-activated neutrophil exocytosis: key roles for interleukin-1-receptor-associated kinase-4 and phosphatidylinositol 3-kinase but not Toll/IL-1 receptor (TIR) domain-containing adaptor inducing IFN-beta (TRIF).
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 169